RNA simultaneous and amplification testing versus the culture method for detecting ureaplasma urealyticum infection
ZHENG Qu
LIU Wei
ZHANG Guo-wei
LI Kai-qiang
WANG Ling-ling
GE Jing-ping
WENG Zhi-qiang
SHANG Xue-jun
Abstract：Objective:To investigate the value of real-titne RNA simultaneous amplification and testing (SAT) in the detection of ureaplasma urealyticum (UU) in the semen of infertile nales and its clinical significance.Methods:We collected semen samples from 542 infertility patients and 120 normal fertile men as controls in the Andrology Clinic of Nanjing General Hospital from March to September 2015.We detected UU infection in the samples using the culture method and SAT technology,respectively.Results:All the UU positive cases (except 4 false positive cases) detected by the culture method were also shown to be positive in SAT.The UU detection rate of SAT was significantly higher than that of the culture method both in the infertility patients (54.1％ vs 19.7％,P ＜ 0.05) and in the normal controls (42.5 vs 12.5 ％,P ＜ 0.05).Conclusion:SAT is a rapid and accurate method for detecting UU infection in semen samples,with a higher sensitivity and accuracy than the culture method,and it can also be used to evaluate the therapeutic effects.However,the culture method has its own advantages,such as low requirement of technical equipment,easy operation,and possibility of drug sensitivity test at the same time.Therefore,SAT and the culture method can be used alternatively accord-ing to the clinical need.
Keywords：real-time RNA simultaneous amplification and testingureaplasma urealyticumculture methodmale infertilitysemen
Publication Date：2017-08-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 717-721 )