Expressions of SLC22A14 and SPAG6 proteins in the ejaculated sperm of idiopathic asthenozoospermia patients
HOU Fang-yuan
LI Yu-shan
YANG Xi-yang
WANG Quan-xian
LIU Jun-jie
WANG Lin-kai
SU Yan-hua
SUN Lin
Abstract：Objective:To investigate the expressions of solute carrier family 22 member 14 (SLC22A14) and sperm-associated antigen 6 (SPAG6) in the sperm of idiopathic asthenospermia men.Methods:We collected semen samples from 50 idiopathic asthenozoospermia patients and another 50 normal sperm donors,purified the sperm by discontinuous density centrifugation on Percoll gradients,and then determined the mRNA and protein expressions of SLC22A14 and SPAG6 by RT-PCR and Western blot,respectively.Results:Compared with the normal controls,the idiopathic asthenozoospermia patients showed significantly decreased mRNA expressions of SLC22A14 (0.77 ± 0.08 vs 0.53 ± 0.10,P ＜0.01) and SPAG6 (0.77 ± 0.06 vs 0.52 ± 0.10,P ＜0.01) and protein expressions of SLC22A14 (0.80 ± 0.09 vs 0.55 ± 0.10,P ＜0.01) andSPAG6 (0.78 ± 0.09 vs0.56 ± 0.09,P ＜ 0.01).Conclusion:The expressions of SLC22A14 and SPAG6 are reduced in the sperm of the patients with idiopathic asthenospermia,which may be one of the important causes of asthenospermia.
Keywords：solute carrier family 22 member 14sperm-associated antigen 6idiopathic asthenozoospermiaspermRT-PCRWestern blot
Publication Date：2017-08-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 703-707 )