Expressions of interleukin-17 and interleukin-8 in the prostatic tissue of the patients with BPH or BPH with inflammation

GAO Rui; LIU Qi-xiang; ZHOU Hui-liang; CAO Lin-sheng; JIANG Tao; TANG Song-xi; DING Yi-lang

doi:10.13263/j.cnki.nja.2017.08.005

Abstract：Objective:To investigate the expressions of interleukin-17 (IL-17) and interleukin-8 (IL-8) in benign prostatic hyperplasia (BPH) and BPH complicated with histological inflammation and their significance.Methods:According to the results of HE staining,we divided 60 cases of BPH treated by transurethral resection of the prostate (TURP) into a BPH group (n =23) and a BPH with inflammation group (n =37).We analyzed the clinical data of the patients and determined the mRNA and protein expressions of IL-17 and IL-8 by immunohistochemistry,real-time fluorescence quantitative PCR,and Western blot,respectively.Results:Compared with the BPH patients complicated with inflammation,the BPH group showed significantly lower International Prostate Symptom Score (IPSS) (29.1 ± 6.2 vs 21.6 ± 3.7),quality of life score (QoL) (5.4 ± 1.3 vs4.4 ± 1.6),postvoid residual urine volume (RUV) ([198.6 ± 15.5] vs [98.2 ± 19.3] ml),prostate volume ([69.2 ± 24.1] vs [49.8 ± 16.5] ml),PSA level ([7.4 ± 1.9] vs [2.8 ± 0.8] μg/L) and serum c-reactive protein content (CRP) ([5.1±2.0] vs [1.5±0.6] mg/L),but a higher maximum urine flow rate (Qmax) ([4.7 ± 2.1] vs [8.2 ± 1.8] ml/s) (all P ＜ 0.05).The former group had a significantly higher incidence rate of urinary retention than the latter (32.4％ [12/37] vs 8.69％ [2/23]),mRNA expressions of IL-17 (0.303 ± 0.076vs0.042 ± 0.019) and IL-8 (0.536 ± 0.059vs0.108 ± 0.025),and protein expressions ofIL-17 (0.88 ± 0.10 vs0.34 ± 0.05) and IL-8 (1.07 ± 0.08 vs0.43 ± 0.04) (allP ＜0.05).Conclusion:The expressions of IL-17 and IL-8 are upregulated in the prostatic tissue of the BPH patients with inflammation,which may play a significant role in the development and progression of BPH.