Role of TGF-β1 in Sertoli cells and tight junction
LOU Jiang-tao
WEI Ren-xiong
YU Lang-lang
CHEN Jian-wei
CUI Yun
Abstract：Objective:To explore the role of TGF-β1 in the proliferation and apoptosis of Sertoli cells and its effect on the expressions of tight junction-related proteins and genes in rats.Methods:Rat Sertoli cells were isolated in vitro,primarily cultured,and divided into groups A (blank control),B (TGF-β1 receptor blocker),C (TGF-β1),and D (TGF-β1 + receptor blocker).The proliferation and apoptosis of the cells were detected by CCK-8 and flow cytometry,respectively.After establishment of the dual-chamber model for the primary culture of Sertoli cells,the trans-epithelia electrical resistance (TER) value was measured and the relative expressions of Occludin,ZO-1 and Claudin Ⅱ determined by RT-PCR and Western blot.Results:The OD value of the proliferation of the Sertoli cells was markedly higher in group C than in groups A and D (0.79 ± 0.04 vs 0.66 ± 0.05 and 0.68 ± 0.02,P ＜0.05),with statistically significant differences among the four groups (F =5.05,P ＜ 0.05).However,no remarkable difference with found among the four groups in the apoptosis rate of the cells (F =1.13,P ＞ 0.05).The TER value was dramatically decreased in group C as compared with groups A and D ([176.37 ± 16.61] vs [281.42 ± 9.83] aud [254.37 ± 13.55] /cm2,P＜0.01),with statistically significant differences among the four groups (F =38.99,P ＜ 0.01).There were no remarkable differences among the four groups in the mRNA expressions of ZO-1 and Claudin Ⅱ (F =0.49 and 0.93,P ＞0.05) or their protein expressions (F =0.28 and 1.31,P ＞ 0.05).Both the mRNA and protein expressions of Oecludin were markedly lower in group C than in A and D (P ＜ 0.01 and P ＜ 0.05),with statistically significant differences among the four groups (F =6.86 and 6.87,P ＜ 0.01).Conclusion:TGF-β1 can promote the proliferation of Sertoli cells in rats and act on the tight junction of the cells by regulating the expression of Occludin.
Keywords：TGF-β1Sertoli celltight junctionOccludinrat
Publication Date：2017-06-02
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 510-516 )