Effects of obesity on global genome DNA methylation and gene imprinting in mouse spermatozoa
ZHU Jin-liang
WU Yin-ling
TANG Wen-hao
TIAN Yuan
GE Shao-qin
LIU Ping
QIAO Jie
Abstract：Objective:To investigate the influence of high fat diet-induced obesity (HFDIO) on the differentially methylated region (DMR) of the imprinted gene and global genome methylation of sperm DNA.Methods:We performed bisulfite sequencing on the DMR of the imprinted gene and global genome methylation of sperm DNA in the mouse model of HFDIO.Results:No statistically significant differences were found between the HFDIO model and normal control mice in MEG3-IG (93.73 vs 97.26％,P =0.252),H19 (98.00vs97.83％,P =0.920),IGF2 (97.34vs96.25％,P =0.166),IGF2R (1.43 vs 1.11％,P =0.695),PEG3 (0.19 vs 0.38％,P =0.537),MEST (0.23 vs 0.68％,P =0.315),NNAT (0.31 vs 0.00％,P =0.134),or SNRPN (1.88 vs 3.13％,P =0.628).A total of 8 942 DMRs were detected across the sperm genome (P ＜ 0.05).Gene functional enrichment analysis indicated that the enriched terms with the largest numbers of genes were the metabolic process (n =1 482),RNA synthesis (n =779),and transcription (n =767).Conclusion:The methylation level underwent no significant change in the DMRs of the imprinted genes from the mice with HFDIO,but the CG methylation of the genes involved in the metabolic process,RNA synthesis and transcription were significantly altered.
Keywords：obesityspermgene imprintingglobal genome methylation
Publication Date：2017-06-02
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：9( 488-496 )