Sperm chromatin structure assay versus sperm chromatin dispersion test in detecting sperm DNA integrity and correlation of sperm DNA fragmentation with semen parameters
WANG Jia-xiong
HAN Mu-tian
SHEN Li-yan
WANG Gai-gai
CHENG Hong-bo
YANG Shen-min
SONG Dan
WANG Wei
LI Hong
SHI Yi-chao
Abstract：Objective:Sperm DNA fragmentation (SDF) is widely used to predict male infertility and the methods of detecting SDF are varied.This study aimed to compare two methods of SDF detection and investigate the correlation between SDF and sperm quality.Methods:Using sperm chromatin structure assay (SCSA) and sperm chromatin dispersion test (SCD),we detected SDF in 108 semen samples collected in the Center of Reproduction and Genetics of Suzhou Municipal Hospital.We compared the results of the two methods and analyzed the correlations of SDF routine semen parameters,sperm morphology and the age of the patients.Results:A significant consistency was found in the SDF index (DFI) between the two methods (P ＜ 0.01).The DFI was correlated negatively with sperm motility,the percentage of progressively motile sperm,and that of morphologically normal sperm (P ＜0.01),but positively with the teratozoospermia index (P ＜0.01 in SCSA and P ＜0.05 in SCD).The DFI measured by SCSA showed a significantly positive correlation with the patients'age (P ＜ 0.01),but not that obtained by SCD.Conclusion:The results of both SCSA and SCD play an important role in predicting sperm quality.As a clinical index,the DFI has a predictive value for male infertility.However,the results of different detecting methods vary widely,which calls for further studies on their standardization.
Keywords：sperm DNA fragmentationsperm chromatin structure assaysperm chromatin dispersion testsperm motilitypercentage of morphologically normal spermage
Publication Date：2017-04-02
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：8( 329-336 )