Expressions of TRPV1 and TRPA1 in the dorsal root ganglion in the rat model of orchialgia
YU Jing-wei
HUANG Jie-hong
L(U) Kun-long
ZHOU Ming-kuan
FENG Xin
TIAN Kun
ZHUANG Jin-tao
ZHOU Wen-liang
DENG Chun-hua
TU Xiang-an
Abstract：Objective:To explore the expressions of transient receptor potential vanilloid 1 (TRPV1) and TRP ankyrin 1 (TRPA1) in the dorsal root ganglion (DRG) and their action mechanisms in the rat model of orchialgia.Methods:The models of orchialgia were established in male SD rats by injection of 2％ acetic acid into the testis.Then the number of spontaneous pain responses and withdrawal latency in the model rats were recorded by behavioral tests and the expressions of TRPV1 and TRPA1 in T13-L1 DRGs determined by RT-qPCR,Western blot and immunofluorescence staining.Results:Compared with the normal control rats,the orchialgia models showed a significant increase in the number of spontaneous pain responses (0.13 ± 0.35 vs 22.63 ± 3.42,P ＜ 0.01) and a decrease in the withdrawal latency at 4 hours after injection ([12.75 ± 1.50] vs [4.85 ± 1.00] s,P ＜ 0.05).The mRNAexpressions of both TRPV1 and TRPA1 were observed in the membrane of the neurons in the DRG,the former increased by 1.77 times and the latter by 1.75 times that of the control (P ＜ 0.05).Conclusion:The expressions of TRPV1 and TRPA1 were up-regulated in the DRG of the rat models of orchialgia,which may be involved in the allodynia and hyperalgesia of the rats.
Keywords：chronic orchialgiadorsal root gangliontransient receptor potential vanilloid 1transient receptor potential ankyrin 1
Publication Date：2017-04-02
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 296-301 )