Comparison of transcription factors repressing epithelial phenotype in two different prostate cancer EMT models and its significance
WU Kai-jie
ZENG Jin
ZHU Guo-dong
ZHANG Dong
XUE Yan
CHEN Yu-le
WANG Xin-yang
HE Da-lin
Abstract：Objective: To screen and compare the specific transcription factors that repress the epithelial phenotype in epitheli-al-mesenchymal transition (EMT) in two different human prostate cancer models LNCaP/HIF1α and ARCaP. Methods: We estab-lished two different prostate cancer EMT models, LNCaP/HIF1α and ARCaP, cultured LNCaP, LNCaP/HIF1α, IF11 and IA8 cells in vitro, and detected the five transcription factors Snail, Slug, ZEB1, SIP1 and Twist1 in these cells by RT-PCR. Results: Different levels of Snail, Slug, ZEB1, SIP1 and Twist1 were detected in both LNCaP and LNCaP/HIF1α cells, with significant differences only in the expressions of Slug and Twist1 between the two cells. The expression of Slug was increased, but that of Twist1 decreased in the LNCaP/HIF1α cells. All the five transcription factors but Twist1 were expressed in both the IF11 and IA8 cells, but only the expres-sions of ZEB1 and Slug were increased significantly in the IA8 cells. Conclusion : There are different mechanisms underlying tran-scriptional regulation in different prostate cancer EMT models. Slug may be one of the key transcription factors involved in the HIF1 α-induced EMT of LNCaP cells, while ZEB1 and Slug may play an important role in repressing the epithelial phenotype of the ARCaP model.
Keywords：prostate cancerLNCaP/HIF1α modelARCaP modelepithclial-mesenchymal transitiontranscription factor
Publication Date：2010-01-31
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 137-141 )