Red clover isoflavones inhibit the proliferation and promote the apoptosis of benign prostatic hyperplasia stromal cells
CHEN Mei-yuan
YAN Shu-chao
YIN Chun-ping
YE Lei
ZHANG Meng-ke
YANG Jun
LIU Ji-hong
Abstract：Objective:To evaluate the effects of red clover isoflavones on the proliferation and apoptosis of human benign prostatic hyperplasia(BPH)stromal cells.Methods:We treated human prostate stromal cells with red clover isoflavones at the concentration of 12.5,25,50 and 100μg/ml,and established a PBS blank control,a dimethyl sulphoxide(DMSO)negative control and four finasteride positive control groups(at the concentration of 12.5,25.0,50.0 and 100.0 μg/ml).We determined the effects of different concentrations of red clover isoflavones on the pmliferafion of the cells by MTT assay and on their apoptosis by Annexin V/PI double stainirig flow cytometry.Results:Red clover isoflavones inhibited the proliferation of the BPH stromal cells by 18.86% at 25.0 μg/ml,compared with 5.17% in the blank control group(P＜0.05),and more obviously at a higher concentration.At 50.0μg/ml,red clover isoflavones exhibited a weaker inhibitory effect than finasteride(28% vs 69.88%,P＜0.05).Annexin V/PI double staining flow cytometry showed that red clover isoflavones at 25.0μl/ml induced the apoptosis of the prostate stromal cells by (18.54±2.5)%,with significant differences from the negative control and blank control(P＜0.01).Conclusion:Red clover isoflavones can inhibit the proliferation and promote the apoptosis of human BPH stromal ceils.
Keywords：red clover isotlavonesbenign prostatic hyperplasiaprostate stromal cellapoptosisproliferation
Publication Date：2010-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 34-39 )