Expressions of phospho-Erk1/2 and phospho-Aktl in the corpus cavernosum of spontaneous hypertensive rats
MAO Jun-biao
XU Chen-xiang
JIANG Rui
Abstract：Objective:To undemtand the expressions of phosphor Erk1/2(P-Erk1/2)and phosphor Akt1(P-Akt1)in the corpus cavernosum of spontaneous hypertensive(SH)and normotensive rats,and to investigate their relationship with penile erectile function.Methods:A series of electric stimuli were applied to the corpus cavernosum nerves of 8 SH rats,the changes of ICP/MAP observed continuously,and the expressions of P-Erk1/2 and P.Akt1 in the corpus cavernosum detected by immunohistochemistry and RT-PCR.Another 8 male WKY rats were taken as controh.Results:ICPl/MAP was significanfly lower iu the SH rats than in the controls(P＜0.01).The mRNA expressions of P-Erkl and P-Erk2 and the protein expression of P-Erk1/2 were significantly increased in the SH group(0.81±0.05,0.91±0.06 and 54.22±10.05),as compared with 0.42±0.04,0.68±0.14 and 7.05±1.45 in the WKY rats(P＜0.05).The mRNA and protein levels of P-Akt exhibited no significant differences between the SH and control groups(0.90±0.05 and 11.17±2.21 versus 0.92±0.06 and 10.91±1.86,P＞0.05).Conclusion:Erectile dysfunction in hypertension patients is associated with the overexpression of P-Erk1/2 in the cavernous tissue.but not obviously correlated with the expression of P-Akt1.
Keywords：phosphor Erk1/2phosphor Akt1corpus cavemosumspontaneous hypertensionerectile functionrat
Publication Date：2010-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 24-28 )