Determination of Sperm DNA Integrity by Improved Sperm Chromatin Structure Assay Test
CAI Jing
LIANG Pei-yan
YIN Biao
LIANG Jin-ming
LIN Qi
ZENG Yong
Abstract：Objective:To search for a simple,quick and accurate method for the sperm DNA integrity test by modifyiag the conven-tional sperm chromatin dispersion(SCD)test,and to investigate the reference value range of normal sperm DNA integrity by the im-proved SCD assay. Methods:We modified the conventional SCD test in its procedures,reagents preparing and utensils to be used,compared the improved method with the conventional one,and detected 293 normal semen samples for sperm DNA integrity by the modified SCD test. Results:An improved SCD test was established,and no statistical differences were found between the results of the modified and conventional methods.The reference value range was determined by the improved SCD assay for normal sperm DNA integrity,which was<32.58% for abnormal sperm DNA integrity. Conclusion:The improved SCD test could detect sperm DNA integrity more quickly,and it reduced the cost in reagents.
Keywords：sperm chromatin dispersion testsperm DNA integrityreference value range
Publication Date：2009-11-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1116-1119 )