Effects of K237 on the Proliferation of PC-3M Cells and mRNA Expressions of Bax and bcl-2

ZHANG Yan-lun; WANG Peng; LU You-yi; HU Nai-gang

Abstract：Objective:To investigate the inhibitory effect of polypeptide K237 on the proliferation of human hormone refractory pros-tare cancer cell line PC-3M and its possible mechanism. Methods:PC-3M cells were divided into three experimental groups and a control,treated with polypoptide K237 at the concentration of 50,100,200 and 0 μmol/L,respectively,for 48 hours.The effects of K237 on the proliferation of different groups of the PC-3M cells were analyzed by MTY,and the mRNA expression levels of bax and bcl-2 were detected by RT-PCR. Results:After polypeptide K237 treatment,the PC-3M cells bacame round,small and less transparent in cytoplasm,and some shed and suspended in the culture medium.The growth inhibition rates of the PC-3M cells were(12.6±0.95)%,(17.8±0.99)%and(27.2±1.12)%in the 50,100 and 200 μmol/L concentration groups.RT-PCR analysis showed that the bax/β-actin values of the 50,100,200 and 0 μmol/L groups were 0.919±0.071,0.971 ±0.083,0.992±0.102 and 0.889±0.067.and the bcl-2/β-actin values of the four groups were 0.896±0.085,0.791±0.084,0.764±0.702 and 0.922±0.097,respectively,both with significant differences between the experimental and the control groups(P<0.01).The mRNA ex-pression of box was upregulated and that of bcl-2 downregulated in a dose-dependent manner. Conclusion:Polypeptide K237 may in-duce apoptosis of PC-3M cells by affecting the expressions of bax and bcl-2,and thus suppress the proliferation of prostate cancer cells.