Efficacy of Interleukin-10 for Experimental Autoimmune Prostatitis

YANG Sen; MU Hai-qi; ZHANG Lei; CHEN Ying-he

Abstract：Objective:To study the efficacy of interleukin-10(IL-10)in the treatment of experimental autoimmune prostatitis(EAP)and its effect on the expressions of tumor necrosis factor-α(TNF-α)and transforming growth factor-β1(TGF-β1)in EAP rat models.Methods:Thirty Wistar rats were equally and randomly divided into a control,an EAP and an IL-10 group.The controls were treated with normal saline,the EAP models were made by iniection of purified prostate protein twice with immune adjuvant,and the IL-10 group included the EAP models subjected to IL-10 intervention.The infiltration of the inflammatory cells of the prostate tissue was de-tected by HE staining,the ultrastructure of the prostate cells and their surrounding cells observed by electron microscopy,and the lcv-els of TNF-α and TGF-β1 in the three groups determined by semi-quantitative RT-PCR assay. Results:The EAP group showed sig-nificantly severer inflammation and higher levels of TNF-α and TGF-β1 in the prostate tissue than the controls(P<0.05).The IL-10 group exhibited significantly lessened inflammatory infiltration of the prostate tissue and decreased levels of TNF-α and TGF-β as compared with the EAP group(P<0.05). Conclusion:IL-10 could relieve inflammatory infiltration ofthe prostate tissue and in-hibit the expressions of TNF-α-and TGF-β1 in EAP rats.which is suggestive of its therapeutic efficacy for autoimmune prostatitis.