Hypoxia Promotes Corpus Cavernosum Smooth Muscle Cell Apoptosis in SD Rats
L(U) Bo-dong
NIAN Jian-hua
HUANG Xiao-jun
ZHANG Shi-geng
GENG qiang
Abstract：Objective: To explore the relationship between hypoxia and the apoptosis of corpus cavernosum smooth muscle cells (CCSMC) in SD rots. Methods: CCSMCs were cultured in vitro and identified by immunohistocbemistry, and then underwent hypoxia interference at the concentration of 1%O_2 for 12, 24, 48 and 72 hours, with normal oxygen concentration as the control. Flow cytometry was used to determine the cycles and apoptosis of the cells. Results: The cultured CCSMCs grew well, positive for antismooth muscle α-actin monoclonal antibody immunohistochemical staining. Flow cytometry showed that the number of CCSMCs in G0/G1 was gradually increased within 48 hours and then decreased, just opposite to the proportion of the S phase cells. But no regular change was found in the proportion of the cells in the G2/M phase. Conclusion: Hypoxia promotes the apoptosis of CCSMCs in a time-dependent manner, to the maximum at 48 hours, and then cell lysis may occur, but with no further apoptasis.
Keywords：corpus cavernosum smooth muscle cellapoptosishypoxiarat
Publication Date：2009-10-26
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 990-993 )