The mTOR Inhibitor Enhances Chemosensitivity of Androgen-Independent Prostate Cancer Cell Line
XIA Jin-sheng
CHEN Xian-guo
ZHUANG Qian-yuan
LIU ji-hong
YE Zhang-qun
Abstract：Objective:To investigate the effect of the mammalian target of rapamycin(mTOR)inhibitor CCI-779 on the chemosensi-tivity of androgen-independent prostate cancer cell line PC-3. Methods:Prostate cancer cells PC-3 were cultured and treated with CCI-779,Paclitaxel and combination of the two.Then the inhibitory effects of the three medications on the growth of the PC-3 cells were determined by MTY.and the their cell cycle and apeptosis were detected by flow cytometry. Results:Compared with the control group,the three medications all significantly inhibited the proliferation of the PC-3 cells,and the combined method even enhanced the effect.Flow cytometry showed that CCI-779 and Paclitaxel blocked the cell cycle mainly in the G1/G2 stage,while the combined medi-cation mainly in the G0/G1 stage.Significantly increased apoptosis of the PC-3 ceUs Was observed in the three medication groups as compared with the control group(P<0.01). Conclusion:CCI-779 Can inhibit the proliferation of PC-3 cells and enhance the the-mosensitivity of prostate cancer.
Keywords：prostate carcinomaandrogen-independentmammalian target of rapamycinCCI-779PC-3 ceBapoptosis
Publication Date：2009-07-19
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 617-620 )