Silencing Nucleostemin Expression Reduces the Proliferation of PC-3 Cells
LIU Ran-lu
XU Yong
ZHANG Zhi-hong
WANG Meng
SUN Jian-tao
ZHANG Yue
LI Sheng-zhi
Abstract：Objective:To detect the expression of the nucleostemin(NS)gene in prostate cancer PC-3,LNCaP and DUl45 cells.and to study the effect of the NS gene on the proliferation of PC-3 cells after its silencing.Methods:The protein and mRNA expres-sions of NS in PC-3,LNCaP and DUl45 cells were respectively detected by immunohistochemical staining and RT_PCR.An NS_specif-ic short-hairpin RNA (shRNA) expression plasmid was used to transfeet the PC-3 cells(NS-shRNA-PC-3),foilowed by observation of the changes of the NS gene and the proliferation and apoptosis of the cells.Results:The NS gene was highly expressed in the three types of cells.After the transfection,the NS expression and the proliferation of the NS-siRNA-PC-3 cells were remarkably redueed,while the percentage of the GO/G1 cells and the early apoptosis of the PC-3 cells obviously increased.A marked decrease was observed in the neoplasm forming ability of the NS-siRNA-PC-3 cells in the nude mice. Conclusion:NS is highly expressed in prostate cancer cells.The proliferation of PC-3 cells is remarkably reduced and the early apoptosis of PC-3 cells increased after silencing the NS geneby NS-specific shRNA.
Keywords：nucleosteminprostate cancerPC-3RNA interference
Publication Date：2009-07-19
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 593-598 )