Effects of YiKangLing on Secretion of Monoclonal Antibodies by Hybridoma Cells
Abstract：Objectives: To explore the effects of both water-phase andethanol-phase extracts of YiKangLing on the secretion of monoclonal antibodies by hybridoma cells. Methods: The water-phase and the ethanol-phase extracts of YiKangLing,a combined Chinese herbal medicine, were respectively added to the cell culture. Then the hybridoma cells able to secrete McAbs against human μ chain,rotavirus of group A common antigen,HCV C33 and cytoplasm antigen of candida albicans were added respectively. After incubation for 96h at 37℃ in 10% CO2 atmosphere, the supernatant cultures were obtained and the levels of McAbs were detected. Results: There existed inhibitory effects of YiKangLing ethanol-phase extract(8.1 ～32.5 g/L),water-phase extract (46.0 ～183.8 g/L),and the water-phase concentrated extract(52.1 ～208.2 g/L)on the McAb secreting ability of hybridoma cells. Conclusions:Chinese medicine YiKangLing extract, its ethanol-phase extract in particular, significantly inhibited the McAb secreting function of antibody producting cells. Ntl J Androl,2001,7（1）:32～34
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Publication Date：2001-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 32-34,37 )