Cloning of Spermatogenesis Related Genes with mRNA Differential Display Method
Abstract：Objectives: To clone the male infertility and spermatogenesisrelated genes by using differential display technique. Methods: Applying mRNA differential display technique,we analyzed mRNA different expression of three kinds of testis tissues (Sertoli-cell-only syndrome,spermatogenic arrest and controller). 17 fragments expressed in both spermatogenic arrest and controller but not in Sertoli-cell-only syndrome were found and all of these fragments were cloned and sequenced.The sequences were compared with the data in GenBank with software Blast 2.0. Results: 3 new ESTs were cloned and shown no homology in GenBank. Conclusions: DDRT-PCR is a useful tool to identify new spermatogenesis related genes.These ESTs may be ESTs of some new spermatogenesis related genes or be new ESTs of some genes. Ntl J Androl,2001,7（1）:20～23
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Publication Date：2001-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 20-23 )