FSH Induced Internalization of FSH Receptor in Transfected and Granulosa Cells
Abstract：Objectives: To characterize the fate of humanfollicle-stimulating hormone(FSH) receptor after being bound by FSH. Methods: A biochemical method able to quickly differentiate the surface bound and internalized hormone was employed to determine the internalization of FSH receptor induced by FSH in cultured porcine granulosa cells and transfected Chinese hamster ovary (CHO) cells expressing recombinant porcine FSH receptor. Results: The results showed that the hormone was slowly internalized, and the internalized radioactivity (acid resistant) reached a maximum 10～12 hours after adding 125I-hFSH. Conclusions: It is possible that the FSHR does not get internalized rapidly under physiological circumstances precisely because the appropriate sequences are absent. Ntl J Androl,2001,7(1):17～19
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Publication Date：2001-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 17-19 )