Effect of orientin on myocardial injury in rats with diabetic cardiomyopathy
XU Shanshan
WANG Xun
TIAN Rong
ZHU Hong
Abstract：Objective To investigate the effect of orientin(Ori)on myocardial injury in DCM rats by regulating the lncRNA NEAT1/miR-34a-5p axis.Methods A rat DCM model was established by combining high-fat diet feeding and streptozotocin(STZ)injection in SPF-grade male Wistar rats.Then,totally 60 DCM rats were randomly divided into DCM group,low-and high-dose Ori groups(Ori-LD and Ori-HD groups),Ori-HD+si-NC group,and Ori-HD+si-NEAT1 group,with 12 rats in each group.In addition,another 12 rats fed with a normal diet were set as the healthy control group(NC group).Echocardiography was applied to assess cardiac function.MDA content,SOD activity,and expression levels of NEAT 1 and miR-34a-5p in heart tissues were determined.Dual-luciferase reporter assay was performed to validate the targeting relationship between NEAT1 and miR-34a-5p.Results Compared with the NC group,the DCM group increased levels of fasting blood glucose,TC and TG,higher apoptotic rate,larger cross-sectional area of myocardial tissue,and elevated MDA content and miR-34a-5p expression level,and lower LVEF,fractional shortening(FS),SOD activity,and NEAT1 expression level(P＜0.05).Ori treatment at both low and high doses decreases in fasting blood glucose,TC,TG,apoptotic rate,cross-sectional area of myocardial tissue,MDA content,and miR-34a-5p expression level,while increases in LVEF,FS,SOD activity,and NEAT1 expression level when compared with the DCM group(P＜0.05).Conversely,the addition of si-NEAT1 elevated fasting blood glucose,TC,TG,apoptotic rate,cross-sectional area of myocardial tissue,MDA content,and miR-34a-5p expression level(P＜0.05),while induced the decreases in LVEF[(62.87±2.70)％vs(79.52±3.59)％],FS[(31.28±1.76)％vs(48.39±3.65)％],SOD activity(162.67±17.38 U/mg vs 218.11±22.42 U/mg)and NEAT1 expression level(0.69±0.08 vs 0.87±0.09)in comparison with simple high-dose Ori treatment(P＜0.05).The relative luciferase activity was reduced in the NEAT1-WT and miR-34a-5p mimics co-transfected cells than the cells co-transfected with miR-NC(P＜0.01).Conclusion Ori can alleviate myocardial injury in DCM rats by inhibiting the expression of lncRNANEAT1 and upregulating the expression of miR-34a-5p.
Keywords：RNAlong noncodingdiabetic cardiomyopathiesheart function testsOrientin
Publication Date：2026-03-15
Online Publishing Date：2026-03-31(First online date of this platform, not the publication date of the document)
Pages：6( 377-382 )