The Relationship Between Signal Peptide-CUB-Epidermal Growth Factor-Like Domain-Containing Protein 1 and Vinculin with Myocardial Injury in Patients with Myocardial Infarction

doi:10.3969/j.issn.1009-0126.2025.10.028

Abstract：Objective To evaluate the predictive value of signal peptide-CUB-epidermal growth factor-like domain-containing protein 1 (SCUBE1) and serum vinculin (Vinculin) for myocardial injury after percutaneous coronary intervention (PCI) in elderly patients with acute myocardial infarction (AMI) using decision curve analysis and dose-response analysis. Methods A total of 68 elderly AMI patients treated in the Department of Critical Care Medicine of Huzhou Traditional Chinese Medicine Hospital, affiliated with Zhejiang University of Traditional Chinese Medicine, from January 2021 to January 2024, were selected as the observation group, and 60 healthy individuals were selected as the control group. According to whether major adverse cardiovascular events (MACE) occurred within 3 months of follow-up, the observation group was divided into MACE group (n=32) and non-MACE group (n=36). Serum levels of SCUBE1 and Vinculin, as well as troponin I (cTnI), creatine kinase isoenzymes-MB (CK-MB), and myoglobin (Myo) were measured. Pearson correlation analysis was used to assess the correlation between SCUBE1, Vinculin, and cTnI, CK-MB, Myo. ROC curve analysis was used to evaluate the predictive value of SCUBE1 and Vinculin for MACE after PCI in elderly AMI patients. Restricted cubic spline plots were used to analyze the dose-response relationship between serum SCUBE1 and Vinculin levels and the risk of MACE after PCI in elderly AMI patients. Decision curve analysis was used to evaluate the predictive value of serum SCUBE1 and Vinculin for MACE after PCI in elderly AMI patients. Results The expression levels of cTnI, CK-MB, Myo, SCUBE1, and Vinculin in the observation group were higher than those in the control group, with statistical significance (P < 0.01). Pearson correlation analysis showed that SCUBE1 and Vinculin were positively correlated with cTnI, CK-MB, and Myo in the observation group. The serum levels of SCUBE1 and Vinculin were higher in the MACE group than in the non-MACE group. ROC curve analysis showed that the area under the curve (AUC) for predicting MACE after PCI in elderly AMI patients by serum SCUBE1 and Vinculin was 0.783 and 0.814, respectively, and the combined prediction AUC was 0.959. There was a nonlinear dose-response relationship between continuous changes in serum SCUBE1 and Vinculin levels and the occurrence of MACE after PCI in elderly AMI patients (r=0.698, r=0.832, P < 0.001). Serum SCUBE1 and Vinculin were significantly positively correlated with the occurrence of MACE after PCI in elderly AMI patients, and the probability of MACE increased with elevated serum SCUBE1 and Vinculin levels. Serum SCUBE1 and Vinculin had certain predictive value for the occurrence of MACE after PCI in elderly AMI patients. Conclusion SCUBE1 and Vinculin are associated with myocardial injury after PCI in elderly AMI patients and have good predictive value.