Effect of matrine on neuroinflammation in ischemic stroke rats

Sun Haidong; Deng Min; Su Xia; Pan Wei

doi:10.3969/j.issn.1009-0126.2024.04.022

Abstract：Objective To investigate the effect of matrine on neuroinflammation in ischemic stroke(IS)rats by regulating the high mobility group protein box1(HMGB1)-receptor for advanced glycation endproducts(RAGE)signaling pathway.Methods Seventy-five SPF-grade rats were ran-domly separated into model group,low-and high-dose matrine groups(10 and 20 mg/kg),high-dose matrine+advanced glycation end products(AGE,RAGE activator)group(20 mg/kg ma-trine+100 mg/kg AGE)and sham operation group,with 15 rats in each group.Thread occlusion method was applied to construct a rat model of middle cerebral artery occlusion(MCAO).Longa score was applied to score neurological deficits.TTC staining was used to measure cerebral infarc-tion,and HE staining was employed to observe pathological changes in hippocampal tissue.ELISA was utilized to detect levels of IL-1β,IL-6,and TNF-α in hippocampal tissue.Western blotting was conducted to determine the protein levels of HMGB1 and RAGE in hippocampal tissue.Results Significantly lower Longa score,smaller cerebral infarct size,decreased levels of IL-1β,IL-6,TNF-α,and reduced protein levels of HMGB1 and RAGE were observed in high-dose matrine group than the model group and low-dose matrine group(P＜0.05).High-dose matrine+AGE treatment resulted in increased Longa score,larger cerebral infarction size,and enhanced levels of IL-1β,IL-6,TNF-α and elevated RAGE protein level when compared with the simple high-dose matrine treatment[2.93±0.30 vs 1.10±0.12,(38.18±4.04)％vs(15.52±1.74)％,78.57±8.33 pg/ml vs 39.27±4.76 pg/ml,203.14±24.39 pg/ml vs 92.45±11.23 pg/ml,243.53±26.81 pg/ml vs 150.49±18.79 pg/ml,0.73±0.07 vs 0.44±0.04,P＜0.05].Conclusion Matrine alleviates neu-roinflammation in IS rats by inhibiting the HMGB1-RAGE signaling pathway.