Value and mechanism of miR-29b in evaluating coronary plaque rupture in patients with coronary syndrome

Wang Kecheng; Cheng Dejun; Hao Jinyao

doi:10.3969/j.issn.1009-0126.2023.12.012

Abstract：Objective To investigate the early warning value of miR-29b in evaluating coronary plaque rupture in patients with stable coronary artery disease(SCAD),and the possible mecha-nism of its causing coronary plaque rupture.Methods A total of 210 SCAD patients treated in our department from April 2020 to April 2022 were selected and divided into PCI group(95 cases)and coronary angiography(CAG)group(115 cases).The expression level of miR-29b in peripheral blood before and after treatment were compared between the two groups.Human peripheral blood mononuclear cells(PBMC)were divided into blank control group,miR-29b group(transfected with miR-29b plasmid)and miR-29b blank group(transfected with miR-29b blank plasmid).The mRNA and protein levels of extracellular matrix metalloproteinase-inducing factor(EMMPRIN),NF-κB,and IL-6 were compared in the 3 groups.Results The expression of miR-29b after PCI was higher than that before PCI,and the expression level after PCI was significantly higher than that after CAG(P＜0.01).ROC curve analysis showed that the cut-off value of preoperative miR-29b in diagnosing coronary plaque rupture was 0.86,and the AUC value was 0.838(95％CI:0.707-0.970).Multivariate logistic regression analysis indicated that preoperative miR-29b was an independent predictor of plaque rupture in the SCAD patients(OR=0.598,95％CI:0.394-0.908,P=0.016).Pearson correlation analysis displayed that preoperative miR-29b was negative-ly correlated with EMMPRIN mRNA level in PBMC(r=-0.575,P＜0.01).In the in vitro experiment,the expression of EMMPRIN,NF-κB and IL-6 at mRNA and protein levels were sig-nificantly lower in the miR-29b group than the blank control group and the miR-29b blank group(P＜0.05).Conclusion Elevated miR-29b level after PCI in SCAD patients is a protective factor for coronary plaque rupture.Its mechanism may be that miR-29b inhibits plaque rupture by down-regulating the expression of EMMPRIN in PBMC.