Effect of L-3-n-butylphthalide on cognitive impairment and expression of N-methyl-D-aspartate receptors in hippocampus of diabetic mice
Abstract：Objective To study the effect of L-3-n-butylphthalide (L NBP) on learning,memory and expression of N-methyl-D-aspartate (NMDA) receptor in hippocampus of diabetic mice.Methods Sixteen diabetic mice were divided into L NBP group (n=8) and DM group (n=8) with 10 littermate db/m mice served as a control group.Six weeks after the animals in L-NBP group were given L-NBP and those in DM group and control group were given vegetable oil by oral gavage.The long-term potentiation (LTP) in hippocampus was recorded during the electrophysiological experiments.The expression of NMDA receptor subunit NR2B mRNA was detected by RT-PCR.Results The escaping latency time was longer,the average number of cross platform explorations was smaller in DM group than in control group (P＜0.05).The learning and memory scores were significantly higher in L-NBP group than in DM group (P＜0.05).The LTP in hippocampus and the expression level of NR2B mRNA in hippocampal CA1 area were significantly lower in DM group and L-NBP group than in control group (136.17％ ± 12.73％,174.17％ ± 20.35％ vs 251.67％±43.78％,P＜0.05;1.02±0.12,1.42±0.12 vs 1.86±0.14,P＜0.05).Conclusion L-NBP improves the leaming and memory of diabetic mice by upregulating the expression of NMDA receptor and LTP in their hippocampal CA1 area.
Keywords：diabetes mellituscognition disordersreceptorsN-methyl-D-aspartatelong-term potentiationmemoryL-3-n-butylpht halide
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1199-1202 )