Inhibitory effect of Ang 1-7 on expression of CD40/CD40L pathway and activated adhesion molecules
Abstract：Objective To study the inhibitory effect of Ang 1-7 on expression of Ang Ⅱ-induced intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) in cultured human umbilical vein endothelial cells (HUVEC) and whether its mechanism is related with the CD40/CD40L pathway.Methods Cultured HUVEC were divided into control group,Ang Ⅱ group,low AngⅡ dose (10 nmol/L) group,medium AngⅡ dose (100 nmol/L) group,high AngⅡ dose (1000 nmol/L) group and blocker (A-779) group.Expressions of ICAM-1,VCAM-1,CD40/CD40L mRNA were detected by RT-PCR.Results The expression levels of ICAM-1,VCAM-1,CD40/CD40L mRNA were significantly higher in Ang Ⅱ group than in control group (P＜0.05)and were significantly lower in low AngⅡ dose group,medium Ang Ⅱ dose group and high Ang Ⅱ dose group than in Ang Ⅱ group (P＜0.05).The expression of ICAM-1 and VCAM-1 decreased by 25％,58％,69％ and 30％,53％,62％,and that of CD40/CD40L mRNA decreased by 35％,48％,61％ and 26 ％,54％,68％.No significant difference was found in the expressions of ICAM-1,VCAM 1,CD40/CD40L mRNA between Ang Ⅱ group and blocker group (P＞0.05).Conclusion Ang 1-7 can inhibit the activation of CD40/CD40L pathway and downregulate the expression of ICAM-1 and VCAM-1 in HUVEC by binding to their specific receptor Mas.
Keywords：angiotensinsangiotensin Ⅱintercellular adhesion molecule-1vascular cell adhesion molecule-1CD40 ligandendothelial cells
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 974-978 )