Effect of artificial cold wave on endothelin and calcitonin gene-related peptide in stroke-prone rats with renovascular hypertension
Abstract：Objective To study the effect of artificial cold wave on endothelin (ET) and calcitonin gene-related peptide (CGRP) in stroke-prone rats with renovascular hypertension.Methods Seventy-two male SD rats were divided into stroke-prone renovascular hypertension group as a model group (n=36) and control group (n=36).The animals in two groups were exposed to artificial cold wave.Brain tissue samples were taken brfore exposure to artificial cold wave,at 3,6 and 12 h during exposure to artificial cold wave,at 12 and 24 h after exposure to artificial cold wave respectively and were then cut into 1.5 mm-thick sections.The expressions of ET,CGRP and ET mRNA were detected by in situ hybridization.Results The expression level of ET and ET mRNA was significantly higher in model group than in control group before exposure to cold wave,at 3,6,12 h during exposure to cold wave,at 12 and 24 h after exposure to cold wave while that of CGRP was significantly lower in model group than in control group before exposure to cold wave and at 24 h after exposure to cold wave (P＜0.05).The expression level of ET and CGRP was significantly higher in model group at 3 and 6 h after exposure to cold wave (P＜0.05).The expression level of ET mRNA was significantly higher in model group at 3,6 and 12 h after exposure to cold wave than before exposure to cold wave (28.00±6.02,27.06±5.46,26.40±4.60 vs 22.81± 4.35,P＜0.05).Conclusion Elevated expression of ET and CGRP in stroke-prone rats with renovascular hypertension is related with stroke when they are exposed to cold wave.
Keywords：hypertensionrenovascularreceptorscalcitonin gene-related peptidechillsstroke
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 867-871 )