Protective effect of breviscapine on cerebral ischemia/reperfusion injury in rats
Abstract：Objective To study the protective effect of breviscapine on cerebral ischemia/reperfusion (I/R) injury in rats.Methods Forty SD rats were randomly divided into sham operation group,normal saline (NS) treatment group (NS+ MCAO),magnesium sulfate treatment group (magnesium sulfate + MCAO),and breviscapine treatment group (breviscapine 75 mg/kg + MCAO),10 in each group.Their neurological function was scored according to Longa occlusion and cylinder test.The infarction size was measured with TTC staining,the water volume in brain tissue was assayed by dry-wet weighing,and the expression of caspase-3 positive cells in ischemic brain tissue was detected with immunohistochemical staining on days 3,7 and 14 after treatment.Results The neurological function score and water volume in brain tissue were significantly high-er while the infarction size and expression level of caspase-3 positive cells were significantly lower in breviscapine treatment group than in NS treatment group and magnesium sulfate treatment group on days 3,7 and 14 after treatment (P＜0.05).Cylinder test showed that the score of asymmetrical upper extremities was significantly lower in breviscapine treatment group than in NS treatment group and magnesium sulfate treatment group on days 3,7 and 14 after treatment (P＜ 0.05).Conclusion Breviscapine injection can effectively improve the neurological function in rats after ischemic stroke by reducing the cerebral I/R injury,alleviating the cerebral edema,and downregulating the caspase-3 expression.
Keywords：brain ischemiareperfusion injuryinfarctionmiddle cerebral arteryprotein kinase CERIGERON BREVISCAPUScaspase 3
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 749-754 )