Effect of hypoxia on expression of N-methyl-D aspartate receptor subunit 1 mRNA in heart of rats
Abstract：Objective To study the effect of hypoxia on expression of N-methyl D-aspartate receptor 1 (NR1) mRNA responsed to cyclic in termittern hypoxia (CIH,10％ O2,12 times/h perSh for 21 days) in heart of rats or persistent hypoxia (1％ O2,5％ CO2,94％ N2) in H9C2 cells.Methods Twelve adult male SD rats were divided into control group (n=6) and CIH group (n=6).Rats underwent CIH at a rate of 12 cycles/h for 8 h/d for 21 days in Oxycycler A84 Hypoxia Chamber,those in control group were exposed to air instead of hypoxia.In a separate experiment,H9C2 cells were exposed to hypoxia for 0 h,1 h,3 h and 6 h,respectively.After exposed,in the left ventricles of the rat hearts and H9C2 cells,NR1 mRNA expression was detected by RT-PCR.Results In the heart of rats,the expression level of NR1 mRNA was 143.65 ± 3.74 in CIH group and 53.01t10.52 in control group (P＜0.05).In the H9C2 cells,the expression level of NR1 mRNA was 18.98±3.73 in 0 h hypoxia group,24.16±6.17 in 1 h hypoxia group,243.68±16.03 in 3 h hypoxia group,and 113.17±6.51 in 6 h hypoxia group,and was significantly higher in 3 h hypoxia group than in the other 3 hypoxia groups (P＜0.05).Conclusion Hypoxia upregulates the NR1 mRNA expression in heart of rats and regulates their response of myocardium to hypoxia.
Keywords：anoxiareceptorsN-methyl-D-aspartateRNAmessengermyocytescardiacsleep apneaobstructive
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 745-748 )