Effect of bone marrow mononuclear cells transplantation in rats following ischemic stroke
Abstract：Objective To study the protective effect of bone marrow mononuclear cells (BMMNC) transplantation in rats following ischemic stroke and its mechanism.Methods Ninety-six SD rats were randomly divided into sham operation group,model group,COX-2 inhibitor group and BMMNC group.Tissue samples were taken at 24 and 72 h and on day 7 after a model of middle cerebral artery occlusion (MCAO) was established by Longa occlusion.The neurological function of rats was scored according to the Zea Longa method.The ischemic stroke size was measured with TTC staining,and expressions of COX-2 and IL-1β were detected by Western blot and immunohistochemical staining.Results The ischemic stroke size was significantly smaller while the expression levels of COX-2 and IL-1β were significantly lower in BMMNC group than in model group at 24 and 72 h and on day 7 after the model was established (0.133±0.032 vs 0.342±0.042,0.141±0.035 vs 0.362±0.062,0.150±0.032 vs 0.330±0.050,P＜0.05;0.213±0.045 vs 0.272±0.055,0.231±0.087 vs 0.331±0.105,0.134±0.030 vs 0.163±0.053,P＜0.05).No significant difference was found in ischemic stroke size and expression levels of COX-2 and IL-1β between BMMNC group and model group (P＞0.05).Conclusion BMMNC transplantation can significantly improve the neurological function of rats following ischemic stroke by inhibiting the immune inflammatory reaction and downregulating the expression levels of COX-2 and IL-1β.
Keywords：brain infarctioncyclooxygenase 2interleukin-1 betabone marrow cells
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 638-641 )