Effect of caveolin-1 on oxLDL-induced aging of macrophages

doi:10.3969/j.issn.1009-0126.2017.06.019

Abstract：Objective To study the effect of caveolin-1 on oxLDL-induced aging of RAW 264.7 macrophages and its mechanism.Methods The aging of RAW 264.7 macrophages was induced with oxLDL (at the concertrations of 20 μg/ml,40 μg/ml,80 μg/ml and 120 μg/ml).Cavevolin-1 protein in RAW 264.7 macrophages was assayed.Expression of cavevolin-1 was detected by interfering RNA.Effect of caveolin-1 on oxLDL (60 μg/ml)-induced aging of RAW 264.7 macrophages was observed.Exprcssion level of p47phox in membrane protein,MDA level in supernatant of RAW 264.7 macrophages,and activity of SOD were measured.Results The aging of RAW 264.7 macrophages and the expression level of caveolin-1 protein in RAW 264.7 macrophages and that of p47phox in membrane protein increased with the increasing oxLDL concentration.The number of aging RAW 264.7 macrophages was less and the expression level of p47phox in membrane protein and the MDA level in supernatant of RAW 264.7 macrophages were lower (7.12± 1.26nmol/mlvs 11.97±1.78 nmol/ml,P＜0.05) while the activity of SOD was higher (79.98±3.94 U/ml vs 50.03 ±6.57 U/ml,P＜0.05) after the expression of caveolin-1 was interfered with RNA than before the expression of caveolin-1 was interfered with RNA.Conclusion Caveolin-1 affects oxLDL-induced aging of RAW 264.7 macrophages by regulating oxidative stress and aging-related atherosclerosis.