Effect of cerebral ischemia preconditioning on receptor of advanced glycosylation end products and TLR4 in rat cerebral cortex
Abstract：Objective To study the effect of cerebral ischemia preconditioning (CIP) on expression of receptor for advanced glycation end (RAGE) products and Toll-like receptor 4 (TLR4) in rat cerebral cortex.Methods One hundred and eight SPF healthy male SD rats were randomly divided into CIP group (n=36),I/R group (n=36),and sham operation group (n=36).After a middle cerebral artery occlusion model of rats was established,6 rats randomly selected from each group were killed on day 1 after reperfusion.The infarction size was measured with TTC staining.The rats were then sacrificed at 12 h and on days 1,2,3 and 7 after reperfusion.Expressions of RAGE products and TLR4 mRNA were detected by RT-PCR.Results The average neural behavioral score was significantly lower in CIP group than in I/R group at 12 h and on days 1,2,3 and 7 after reperfusion (P＜0.05,P＜0.01).The infarction size was significantly smaller in CIP group than in I/R group on day 1 after reperfusion (19.04％±1.65％ vs 34.55 ％ ±4.78％,P＜0.05).The expression levels of RAGE products and TLR4 mRNA were significantly higher in CIP group and I/R group than in sham operation group (P＜0.05) and reached their peak on day 1 after reperfusion and then gradually decreased with the prolonged time of reperfusion.The expression levels of RAGE products and TLR4 mRNA were significantly lower in CIP group than in I/R group (P＜0.05).Conclusion CIP increases the tolerance to cerebral ischemia by downregulating the expression of RAGE products and TLR4 mRNA in rats following I/R injury.
Keywords：ischemic preconditioningcerebral cortexglycosylation end productsadvancedreperfusion injuryhigh mobility group proteins
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 83-86 )