Role of T cells activation in Meprin-α-mediated formation of atherosclerosis
Abstract：Objective To study the role of T cells activation in Meprin-α-mediated formation of atherosclerosis and its mechanism.Methods Thirty 6 weeks old apoE/ mice were randomly divided into model group,L-MeplA group and control group (10 in each group).The animals were given PBS,L-MeplA virus,and control virus plasmid after they were fed with a high fat diet for 10 weeks.Their aortic atherosclerotic plaques were observed with HE staining,peripheral T cell subgroups were detected by flow cytometry,and inflammatory cytokine secretion was tested by ELISA.Results The size of atherosclerotic plaques was significantly larger,the number of CD3 and CD4 T cells was significantly greater,and the serum IL-6 and IFN-γ levels were significantly higher in L-MeplA group than in model group (41.3±6.8 pg/ml vs 20.2±4.1 pg/ml,30.3±4.2 pg/ml vs 11.3±2.1 pg/ml,P＜0.05).No significant difference was found in the number of CD3 and CD4 T cells,serum IL-4,IL-5,IL-10 and TGF-β levels between model group and control group (P＞0.05).Conclusion The role of Meprin-α in mediating atherosclerosis is related with immune dysfunction.Meprin-α accelerates inflammatory response by mediating the Th1 cells activation,thus leading to formation of atherosclerosis.
Keywords：atherosclerosisT-lymphocyte subsetsmetalloproteasesinterleukin-6
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 74-77 )