Effect of oxLDL on expression of shear stress-induced endothelial NO synthase in human aortic endothelial cells
Abstract：Objective To study the effect of oxLDL on expression of shear stress (SS)-induced endothelial NO synthase (Enos) in human aortic endothelial cells.Methods Culturaled human aortic endothelial cells were divided into control group and experimental group.SS was enforced in two groups with a precision infusion pump and experimental group was stimulated with 100μg/ml oxLDL.Each of the two groups was divided into SS-free group (n=5),low SS group (n=5) and high SS group (n=5) with a SS of 0 dyn/cm2,4 dyn/cm2,and 15 dyn/cm2 respectively.Expressions of eNOs Mrna and protein were detected by RT-PCR and Western blot respectively.Serum NO level was measured using nitrate reductase method.Results The serum NO level and Enos Mrna and protein expression levels were significantly lower in low SS group than in Ssfree group,significantly higher in high SS group than in SS-free group and low SS group,the serum NO level and Enos Mrna and protein expression level were significantly lower in SS-free group,low SS group and high SS group than in control group.The serum NO level was significantly lower in low SS group than in SS-free group.Conclusion oxLDL reduces high SS-induced activity of endothelial cells and aggravates low SS-induced dysfunction of endothelial cells.
Keywords：lipoproteinsLDLendothelial cellsnitric oxide synthaseRNAmessenger
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 13-15 )