Effect of methycobal on apoptosis and expression of cytochrome C in brain tissue of rats following ischemia reperfusion injury
Abstract：Objective To study the effect of methycobal on expression of cytochrome C in brain tis‐sue of rats following ischemia reperfusion (I/R) injury .Methods One hundred and thirty‐two Wistar rats were randomly divided into sham operation group (n=24) ,model group (n=27) ,ni‐modipine treatment group (n=27) ,low methycobal dose treatment group (n=27) ,high methyco‐bal dose treatment group (n=27) .A rat I/R injury model was established by Longa occlusion of the middle cerebral artery .Apoptosis and expression of cytochrome C in brain tissue of rats were detected by TUNEL and Western blot respectively at 6 ,12 ,24 h .Results The number of positive cells was significantly smaller in nimodipine treatment group ,low methycobal dose treatment group and high methycobal dose treatment group than in model group at 6 ,12 ,24 h (P<0 .01) . The expression level of cytochrome C was significantly higher in model group than in sham opera‐tion group ,significantly lower in nimodipine treatment group ,low methycobal dose treatment group and high methycobal dose treatment group than in model group at 6 ,12 ,24 h ( P<0 .05 , P<0 .01)and in high methycobal dose treatment group than in low methycobal dose treatment groupat6,12h(0.466±0.046 vs0.617±0.033,P<0.01 ;0.163±0.081 vs0.552±0.132,P<0 .05) .Conclusion Methycobal protects brain tissue of rats against I/R injury by downregulating the expression of cytochrome C .
Keywords：cytochromes Capoptosisnimodipinebrain ischemiareperfusion injurymethycobal
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1193-1196 )