Temporal and spatial distribution of lysine-specific demethylase 1 in rats following transient global ischemia
Abstract：Objective To study the temporal and spatial distribution of lysine‐specific demethylase 1 (LSD1)in rats following transient global ischemia .Methods One hundred and twenty‐six adult SD rats were included in this study .Sixty‐three of the animals were randomly divided into 8 (1 ,6 , 12 h and 1 ,3 ,7 ,14 ,30 d) experimental groups (7 in each group) and control group (n=7) .Ex‐pression of LSD1 in different sites of rat brain was detected by immunohistochemistry and West‐ern blot ,respectively ,after a model of transient global ischemia was established .Results Immu‐nohistochemistry and Western blot showed that LSD1 was mainly distributed in hippocampus and frontal cortex of rats .The number of positive immunoreactive cells to LSD1 in experimental groups increased significantly at 1 h and reached its peak at 6 h after I/R ,then decreased slowly and reached its lowest point at 12 h after I/R ,but was significantly greater in dentate gyrus and CA1 of enperimental groups than in those of control group (P<0 .05) .However ,the number of positive immunoreactive cells to LSD1 in dentate gyrus ,CAI and frontal cortex was significantly greater in experimental groups than in control group at 12 h after I/R (P<0 .01) ,reached its sec‐ond peak on days 1 ,3 ,7 ,then decreased slowly and returned to that in control group on day 30 af‐ter after I/R .Conclusion LSD1 in dentate gyrus ,CA1 ,and frontal cortex of rats plays an impor‐tant role in regulating the pathways following cerebral ischemia .
Keywords：brain ischemiahistone-lysine N-methyltransferaseischemic attacktransientimmuno-histochemistryhippocampusreperfusion injury
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 968-971 )