Effect of Danlong Xingnao Recipe on proliferation of neural stem cells in rats and its mechanism
Abstract：Objective To study the effect of Danlong Xingnao Recipe (DLXNR) on proliferation of neural stem cells (NSC) and expression of NGF ,bFGF ,GDNF after reperfusion in rats following cerebral ischemia .Methods Sixty SD rats were randomly divided into sham operation group , model group ,3 .2 mg/kg edaravone treatment group ,3 .7 mg/kg DLXNR treatment group ,7 .48 g/kg DLXNR treatment group and 14 .8 g/kg DLXNR treatment group (10 in each group) .A rat cerebral ischemia/reperfusion model was induced by middle cerebral artery occlusion and recanali-zation .Proliferation of NSC was detected by Brdu incorporating after the rats were treated for 7 days .Expressions of NGF ,bFGF and GDNF were detected by immunohistochemistry .Results The expression level of NGF ,bFGF and GDNF was significantly lower in model group than in sham operation group and in 7 .48 g/kg DLXNR treatment group and 14 .8 g/kg DLXNR treat-ment group than in model group (P< 0 .05) .The number of proliferated NSC was significantly greater in 3 DLXNR treatment groups than in model group (P<0 .05) .Conclusion DLXNR can stimulate the proliferation of NSC by up-regulating the expressin of NGF ,bFGF and GDNF .
Keywords：brain ischemiareperfusionstem cellscell proliferationnerve grow th factorfibro-blast growth factor 2glial cell line-derived neurotrophic factorBLOOD ACT STASIS REMOV AGENTS
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1090-1093 )