Expression of adiponectin receptor in a mouse transient cerebral ischemia model
Abstract：Objective To study the mechanism of adiponectin receptor(APNR) in protecting mouse brain by observing its expression in a mouse transient cerebral ischemia model .Methods Sixty CD-1 mice that underwent tMCAO were included in this study .Their brain tissue samples were taken before ischemia .Expression of APNR in mouse brain tissue samples taken before ischemia , and at 1 and 4 h ,and on days 3 and 7 after reperfusion was detected by RT-PCR ,Western blot and immunohistochemistry ,respectively .Results The expression level of APNR1 protein and mRNA was significantly higher in ischemic tissue at 4 h and on days 3 and 7 after reperfusion than before ischemia(P<0 .05 ,P<0 .01) .The expression level of APNR1 protein was similar in central is-chemic area and peripheral ischemic area .APNR1 was expressed in endothelial cells ,astrocytes and neurons .APNR2 was not expressed in brain tissue before and after ischemia .Conclusion The expression of APNR1 is up-regulated in ischemic brain tissue following ischemia-reperfusion inju-ry .Adiponectin may exert its brain protective effect via APNR1 .
Keywords：adiponectinreceptorsbrain ischemiareperfusion injury
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1086-1089 )