Effect of intermittent hypoxia on expression of nerve cell autophagy-related protein in hippocampus of rats
Abstract：Objective To study the effect of intermittent hypoxia (IH) on expression of nerve cell autophagy-related protein in hippocampus of rats by simulating the features of OSA HS .Methods Seventy-two male Wistar rats were randomly divided into control group (n=36)and 5% IH group (n=36) .Rats in control group received compressed air and those in 5% IH group were exposed to IH for 7 h each day .On days 1 ,3 ,5 ,7 ,10 ,14 after the model was established ,ultrastructure of rat hippocampal CA1 pyramidal cell mitochondria was observed under transmission electron micro-scope .Expression of Beclin-1 and LC3 was detected by immunohistochemistry assay .Results The ultrastructure change of rat hippocampal CA1 pyramidal cell mitochondria was more significant in 5% IH group than in control group ,and mitochondria autophagosome formation was observed on day 3 .The expression level of Beclin-1 and LC3 protein was significantly higher in 5% IH group than in control group (P<0 .05) ,reached its peak on day 10 (P<0 .05) and began to decrease on day 14 (P<0 .05) .Conclusion Early IH can induce nerve cell autophagy and expression of auto-phagy-related Beclin-1 and LC3 protein in hippocampus of rats .
Keywords：sleep apneaobstructiveanoxiahippocampusneuronsmitochondriaautophagymi-crotubule-associated proteins
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1082-1085 )