Effect ofβ3 adrenoceptor activation on expression of pancreatic angiotensin receptor in apoE/mice
Abstract：Objective To study the effect ofβ3 adrenoceptor (β3-AR)activation on serum blood glu-cose and insulin level and expression of pancreatic angiotensin receptor (ATR) in apoE/mice . Methods Ten wild-type C57BL/6J mice at the age of 10 weeks served as a normal control group (group A)and 50 apoE/mice at the age of 10 weeks fed with high fat diet to 36 weeks old were randomly divided into hyperlipidaemia model group (group B ) ,atorvastatin treatment group (group C) ,low β3-AR agonist dose group (group D) ,high β3-AR agonist dose group (group E) andβ3-AR antagonist group (group F) ,10 in each group .The mice were treated withβ3-AR ago-nist and antagonist for 12 weeks .Serum blood glucose and insulin levels in 48 weeks old mice were measured using an automatic biochemical detector .Expression of AT1 R and AT2 R in pancre-atic tissues of mice was detected by RT-PCR and Western blot ,respectively .Results The serum blood glucose and insulin levels were significantly higher ,the expression level of AT1 R was sig-nificantly higher while that of AT2R was significantly lower in group B than in group A (P<0.01) .The serum blood glucose and insulin levels were significantly lower ,the expression level of AT1 R was significantly lower while that of AT2 R was significantly higher in groups D and E than in group B ( P<0 .01) .Conclusion Long-term use of β3-AR agonist can improve blood glucose metabolism in apoE/mice by down-regulating the AT1 R expression and up-regulating the AT2 R expression ,indicating that β3-AR interacts with AT1 R and AT2 R .
Keywords：apolipoproteins Emiceknockoutblood glucoseinsulinreceptorsangiotensinadren-ergic beta-agonists
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1069-1072 )