Effect of adenovirus-mediated N-ethylamaleimide sensitive factor siRNA on cardiac function in rats with myocardial infarction
Abstract：Objective To study the effect of adenovirus-mediated N-ethylamaleimide sensitive fac-tor (NSF) siRNA on cardiac function in rats with myocardial infarction (MI) .Methods Thirty-six adult SD rats were randomly divided into experimental group ,control group and normal saline group (12 in each group) .Two weeks after NSF siRNA recombinant adenovirus vector was injec-ted into the infarcted part on the left ventricular wall ,the LVEF was tested by noninvasive ultra-sonic cardiography ,the LVEDP and +dp/dtmax were measured .The rat heart was removed and cut into serial sections which were stained with TTC .The MI size was observed .Results Two weeks after the NSF siRNA recombinant adenovirus vector was injected ,the LVEF was significantly higher while the LVEDP was significantly lower while the +dp/dtmax was significantly higher in experimental group than in control group and normal saline group ( P< 0 .05 ) .No significant difference was observed in MI size although it was smaller in experimental group than in control group and normal saline group ( P> 0 .05) .Conclusion Injection of adenovirus-mediated NSF siRNA expression vector into the MI can significantly improve the cardiac function in rats with acute MI .However ,it shows no effect on the MI size .
Keywords：N-ethylmaleimide-sensitive proteinsRNAsmall interferingmyocardial infarction
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 967-970 )