Rat left and right middle cerebral artery ischemia reperfusion models
Abstract：Objective To compare the rat left and right cerebral middle artery (MCA ) ischemia/reperfusion (I/R) models .Methods Forty-eight male Wistar rats were randomly divided into left MCA I/R group and right MCA I/R group (24 in each group) .The MCA was occluded for 2 h by Longa occlusion and then reperfused .Neurological function of rats was assessed .Rat brain tissue samples were stained with TTC and HE .Ischemic stroke volume was measured and brain lesions were observed under light microscope .Results The neurological scores at 24 ,48 and 72 h were significantly lower in left MCA I/R group than in right MCA I/R group (P<0 .05) .TTC staining demonstrated that the ischemia was severer in left MCA I/R group than in right MCA I/R group (P<0 .05) .HE-stained hippocampus tissue showed that the number of lost neurons was greater , the cells were worse dearranged ,and the ischemic stroke volume was larger (102 .1 ± 8 .8 mm3 vs 97.0 ± 11 .2 mm3 ) in left MCA I/R group than in right MCA I/R group (P<0 .05) .Conclusion T he neurological defect is severer and the ischemic stroke volume is larger in the dominant hemi-sphere than in the non-dominant hemisphere of rats following MCA occlusion .The repeatability of rat focal ischemia model is better and more reliable in dominant hemisphere than in non-dominant hemisphere .
Keywords：middle cerebral arterybrain ischemiareperfusionmodelsanimal
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 863-866 )