Effect of trimetazidine and ramipril on myocardial cell apoptosis and Caspase-3 expression in heart failure rats

doi:10.3969/j.issn.1009-0126.2014.08.022

Abstract：Objective To study the effect of trimetazidine or ramipril alone and their combination on myocardial cell apoptosis and Caspase-3 expression in chronic heart failure rats .Methods Fifty male Wistar rats were randomly divided into sham operation group ,model group ,trimetazidine group ,ramipril group and combined treatment (trimetazidine and ramipril ) group (10 in each group) .A chronic heart failure model was established by suprarenal aortic constriction .LVEDP and ± dp/dtmax were recorded ,AI of myocardial cells was assayed by TUNEL ,expressions of Caspase-3 protein and mRNA were detected by SP immunohistochemistry and RT-PCR respec-tively in different groups .Results The LVEDP ,AI and the expression levels of Caspase-3 protein and mRNA were significantly lower in trimetazidine group ,ramipril group and combined treat-ment group than in model group (0 .14 ± 0 .01 vs 0 .22 ± 0 .02 ,0 .14 ± 0 .02 vs 0 .54 ± 0 .02 ,0.08 ± 0 .02 vs 0 .54 ± 0 .02 ,and 0 .32 ± 0 .02 ,0 .91 ± 0 .02 ,P<0 .01) while the ± dp/dtmax was significant-ly higher in trimetazidine group ,ramipril group and combined treatment group than in model group .Conclusion Trimetazidine or ramipril alone and their combination can effectively improve cardiac function and inhibit myocardial cell apoptosis in chronic heart failure rats .T he therapeutic effect of combined trimetazidine and ramipril is better than that of trimetazidine or ramipril alone .