Construction and identification of rat miR-22 adenovirus vector
Abstract：Objective To construct and identify the rat miR-22 adenovirus vector for further stud-ying the role of miR-22 in myocardial ischemia/reperfusion injury and its molecular mechanism . Methods Target gene sequences were synthesized .The miR-22 adenovirus vector was construc-ted by amplifying and digesting the target gene and linking the digested GV 202 vector to the tar-get gene .The positive clones achieved by transfecting the DH5a cells into the constructed vector were identified by digesting and sequencing ,respectively .The recombinant adenovirus with a titer of 1 × 109 PFU/ml obtained by transfecting the HEK293 cells into the constructed miR-22 adeno-virus vector and adenovirus package plasmids with Lipofectin 2000 was amplified and puried . Results The rat miR-22 recombinant adenovirus vector with a titer of 1 × 109 PFU/ml was suc-cessfully constructed .Conclusion The successfully constructed miR-22 recombinant adenovirus vector can be used in studying the role of miR-22 in myocardial ischemia/reperfusion injury and its molecular mechanism .
Keywords：adenoviridaemicroRNAsmyocardial reperfusion injuryplasmidstransfectionlipo-somes
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 853-855 )