Adherent culture and identification of smooth muscle cells isolated from SD rat thoracic aorta
Abstract：Objective To identify the vascular smooth muscle cells (VSMC) isolated from SD rat thoracic aorta after adherent culture .Methods The thoracic aorta was isolated from SD rats un-der sterile conditions .The VSMC were isolated from the SD rat thoracic aorta ,primarily cultured 4-6 h later with DMEM containing 20% FCS for two weeks ,and identified with immunoflures-cence staining .Results The VSMC grew out on days 5-7 ,became nearly 80% confluent on days 10-14 after adherent culture ,and could thus be passaged .The in vitro cultured VSMC could be passaged to the 20th generation and grew in a typical“peak valley”mode .Immunoflurescence stai-ning showed that their purity was 95% .Conclusion Adherent culture is a stable ,simple and con-venient procedure for VSMC isolated from SD rat aorta with a high purity .Immunoflurescence staining can effectively identify VSMC .
Keywords：aortathoracicmusclesmoothvascularmyocytessmooth musclecell fusiontissue culture techniquesfluoroimmunoassay
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 850-852 )