Effect ofβ3-AR activation on expression of angiotensin receptors in lungs of apoE/knockout mice
Abstract：Objective To study the effect of β3-AR activation on expression of angiotensin receptors in lungs of aged apoE/knockout mice .Methods Ten C57BL/6J mice served as a control group , and 40 apoE/knockout mice were randomly divided into hyperlipidemia model group ,low β3-AR agonist group (low dose group) ,highβ3-AR agonist group (high dose group) andβ3-AR antago-nist group (antagonist group) .The animals underwent drug treatment for 12 weeks .Their serum TC and VLDL/LDL-C levels were measured by biochemical analyzer .The β3-AR ,AT1 R and AT2 R mRNA and protein expressions in lung were detected by RT-PCR or Western blot .Results The AT1 R mRNA and protein expression levels were higher while the AT 2 R mRNA and β3-AR protein expression levels were significantly lower in model and antagonist groups than in control group (P<0 .05) .Compared with model group ,the AT1 R expression were down-regulated and AT2 R andβ3-AR were up-regulated in the low dose and high dose groups .Conclusion β3-AR ac-tivation improves lipid metabolism disorder and affects expression levels of β3-AR and ATR sub-types in lungs of aged apoE/knockout mice ,indicating that the sympathetic adrenergic system in-teracts with the angiotensin system in the lungs of aged apoE/knockout mice .These interaction may be associated with the maintenance of normal lung function in hyperlipidemia .
Keywords：receptorsadrenergicbeta-3apolipoproteins Emiceknockoutangiotensin
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 846-849 )