Role of kynurenine metabolic pathway in rats with cognitive impairment induced by chronic cerebral hypoperfusion
Abstract：Objective To study the features of kynurenine (KYN )pathway metabolites in hippo-campus of rats with cognitive impairment induced by chronic cerebral hypoperfusion by observing the expressions of IFN-γ,TNF-α,iNOS ,IDO and KYN .Methods Thirty Wistar rats were ran-domly divided into sham operation group and bilateral carotid artery permanent ligation group (15 in each group) .A rat model of chronic cerebral hypoperfusion was established .Spatial learning and memory ability in bilateral carotid artery permanent ligation group was tested in Morris water maze .Expressions of iNOS ,IFN-γ ,TNFαin hippocampal CA1 area were detected by immunohisto-chemistry and ELISA ,respectively .Results The escape latency period was significantly longer while the platform quadrant swimming distance was significantly shorter in bilateral carotid artery permanent ligation group than in sham operation group (P<0 .05) .Immunohistochemical staining showed that the expression levels of iNOS ,INF-γ ,TNF-α,IDO and KYN were significantly high-er in bilateral carotid artery permanent ligation group than in sham operation group (P<0 .05) . Conclusion The pathophysiological process in rats with chronic cerebral perfusion includes in-flammatory response which may stimulate the over-expression of IDO and KYN .Accumulated KYN and IDO may be involved in cognitive impairment induced by chronic cerebral hypoperfusion .
Keywords：kynureninecognition disordershippocampusinterferon type Ⅱtumor necrosis fac-tor-alphanitric-oxide synthase
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 637-640 )