Effect of small G protein Rad gene on cardiac hypertrophy
Abstract：Objective To provide the theoretical evidence for early treatment of myocardial hyper-trophy by studying the effect of Rad gene on myocardial hypertrophy .Methods Adenovirus vec-tors were constructed for over and low expression of Rad .In vitro cultured suckling rat myocardi-al cells were divided into blank control group ,Ad-GFP group ,Ad-RNAi group ,Ad-Rad group ,hy-pertrophy model group (induced by cardiotrophin 1) ,hypertrophy+ Ad-GFP group ,hypertrophy+ Ad-RNAi group ,hypertrophy+ Ad-Rad group .Expressions of Rad and ANF mRNA and pro-tein were detected 48 h after transfection .Results The expression level of ANF protein was sig-nificantly higher in hypertrophy model group than in blank control group ,significantly lower in hypertrophy+Ad-Rad group than in hypertrophy+Ad-GFP group and significantly higher in hy-pertrophy+ Ad-RNAi group than in hypertrophy + Ad-GFP group (P< 0 .05) .The expression level of Rad mRNA and protein was significantly lower in hypertrophy model group than in blank control group ,significantly higher in hypertrophy+Ad-Rad group than in hypertrophy+Ad-GFP group and significantly lower in hypertrophy + Ad-RNAi group than in hypertrophy + Ad-GFP group (P<0 .05) .Conclusion Rad gene can inhibit cardiotrophin 1-induced myocardial hypertro-phy at cellular level .
Keywords：cardiomyopathyhypertrophicmyocytescardiacRNAmessengermonomeric GTP-binding proteins
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 630-632 )