Effect of β3-AR agonists on expression of PCSK9 and LDLR in apoE gene knock-out mice

doi:10.3969/j.issn.1009-0126.2014.06.020

Abstract：Objective To study the effect of β3-AR agonists on expression of PCSK9 and LDLR in elderly apoE gene knock-out mice .Methods Ten male C57BL/6 mice aged 10 weeks served as group A and 40 apoE gene knock-out mice fed with high fat diet to 36 weeks old were randomly divided into high fat model group (group B) ,positive atrovastatin control group (group C) ,small doseβ3-AR agonist group (group D) and large dose β3-AR agonist group (group E) ,10 in each group .The animals were treated with atorvastatin and β3-AR agonists for 12 weeks .Serum levels of TC ,TG ,VLDL/LDL-C ,HDL-C were measured with the Beckman CX7 automatic biochemical analyzer and expression of PCSK9 and LDLR was detected by Western blot .Results The serum TC and VLDL/LDL-C levels were significantly lower in groups C ,D and E than in group B ,the expression level of LDLR was significantly higher in groups C ,B and D than in group E while that of PCSK9 was significantly higher in group C than in groups B ,D and E (P<0 .05 ,P<0 .01) .The serum TG level was significantly lower in groups D and E than in group C ,the expression level of LDLR was significantly lower while the serum HDL-C level was significantly higher in group D than in group C (P<0 .05 ,P<0 .01) .Conclusion β3-AR agonist decreases TC ,VLDL/LDL-C , TG levels and expression of PCSK9 ,and increases LDLR and HDL-C level ,which are one of its anti-atherosclerosis mechanisms in elderly apoE-/-mice .