PTEN expression in CD 4 T lymphocytes of unstable angina pectoris patients during perioperative PCI
Abstract：Objective To study the PTEN expression in CD4 T lymphocytes of unstable angina pectoris patients during perioperative PCI .Methods Forty-two unstable angina pectoris patients who were going to undergo PCI were enrolled in this study .Peripheral blood samples were taken before and 16-24 h after PCI .CD4 T lymphocytes were isolated using the magnetic cell sorting system .Expression of PTEN mRNA and protein was detected by RFQ-PCR and Western blot re-spectively .Serum levels of TNF-α and IL-10 were measured by ELISA .Results The incidence of cTnI was 45 .4% higher while the expression level of PTEN mRNA and protein was significantly lower after PCI than before PCI .The serum TNF-αlevel was significantly higher while the serum IL-10 level was significantly lower after PCI than before PCI (P<0 .05 ,P<0 .01) .Linear correla-tion analysis showed that PTEN protein expression was negatively related with serum TNF-αlev-el (r= -0 .874 ,P<0 .01) and positively related with serum IL-10 level (r= 0 .732 ,P<0 .05) . Conclusion PCI promotes secretion of inflammatory factors by down-regulating PTEN expres-sion ,thus inducing myocardial inflammation injury .
Keywords：anginaunstableCD4-positive T-lymphocytestumor necrosis factor-alphainterleukin-10troponin IPT EN phosphohydrolase
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 577-579 )