Expression of regeneration-related factor in rats with intracerebral hemorrhage after transplantation of neural stem cells
Abstract：Objective To observe the expression of BDNF and VEGF and vasculogenesis in rats with intracerebral hemorrhage(ICH) after transplantation of NSCs. Method 140 SD rats were di-vided randomly into sham operated group(group A), model group(group B), NSCs culture medi-um transplanted group(group C) and NSCs transplanted group(group D). NSCs were transplan-ted into the subventricular zone in rat models of ICH,The expression changes of BDNF,VEGF,microvessel density(MVD) at 6 h and on days 1,4,7,14,21 and 28 after ICH was observed by means of immunohistochemical technique. Results Compared with group A, the expression of BDNF peaked on first day in groups B and C,and the expression peak was delayed to 4th day in group D,but maintained higher expression than group A on 28th day (P<0.05). At all time points,positive cell counts between group D and group B had significant difference (P<0.05).On days 4,7,14,21 and 28,positive cell counts between group D and group C had significant difference (P < 0.05). The expression levels of VEGF and MVD peaked on first day in groups B and C,and were higher in group C than in group B on days 1,4. Group D had double expression peaks on 4th day and 14th day,and maintained higher expression than in groups B,C on 28th day (P < 0.05). VEGF showed positive correlation with MVD (r=0.911 ,P<0.05). Conclusions The high expression of BDNF and VEGF is maintained for a long time and MVD increases in rats with cerebral hemorrhage after transplantation of NSCs.
Keywords：cerebral hemorrhagestem cell transplantationbrain-derived neurotrophic factorvas-cular endothelial growth factor Aimmunohistochemistry
Publication Date：2009-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 621-624 )